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Anti-LYSOZYME (RABBIT) Antibody
Lysozyme Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Chicken |
| Reactivity | Chicken |
| Clonality | Polyclonal |
Application 
| WB, E, IP, I, LCI |
| Application Note | Anti-Lysozyme antibody has been tested by western blot and ELISA. This product has been assayed against 1.0 ug of Lysozyme [Hen Egg White] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. |
| Physical State | Lyophilized |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Lysozyme [Hen Egg White] |
| Reconstitution Volume | 2.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 396218 |
|---|---|
| Other Names | 396218 |
| Purity | Anti-Lysozyme antibody is an antiserum prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Lysozyme [Hen Egg White]. Cross reactivity against Lysozyme from other tissues and species may occur but have not been specifically determined. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | LYZ |
|---|---|
| Function | Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. |
| Cellular Location | Secreted. |
| Tissue Location | In the egg white and polymorphonuclear leukocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Lysozyme antibody targets lysozyme protein. Lysozyme (also called muramidase and 1,4-beta-N-acetylmuramidase ) is abundant in a number of secretions, such as tears, saliva, human milk, and mucus. The enzyme functions by attacking peptidoglycans (found in the cell walls of bacteria, especially Gram-positive bacteria) and hydrolyzing the glycosidic bond that connects N-acetylmuramic acid with the fourth carbon atom of N-acetylglucosamine. The anti-Lysozyme antibody is suitable for detection of lysozyme from a number of different sources.
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