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Anti-PEPSIN (GOAT) Antibody
Pepsin Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Goat |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Swine |
| Reactivity | Pig |
| Clonality | Polyclonal |
Application 
| WB, IHC, E, IP, I, LCI |
| Application Note | Anti-Pepsin antibody has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Physical State | Lyophilized |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. |
| Reconstitution Volume | 100 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Preservative | 0.01% (w/v) Sodium Azide |
| Other Names | 396892 |
|---|---|
| Purity | Anti-PEPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | PGA |
|---|---|
| Function | Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. |
| Cellular Location | Secreted. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Pepsin antibody detects Pepsin protein. Pepsin is an enzyme whose pepsinogen is released by the chief cells in the stomach and degrades food proteins into peptides. Pepsin belongs to the peptidase A1 family and is one of three principal proteolytic enzymes in the digestive system. Pepsin is most efficient in cleaving peptide bonds between hydrophobic and preferably aromatic amino acids such as phenylalanine, tryptophan, and tyrosine at positions P1 and P1'. Anti-Pepsin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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