Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Anti-Esrp-2 (MOUSE) Monoclonal Antibody
Esrp-2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Mouse |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Monoclonal |
Application 
| WB, E, I, LCI |
| Application Note | Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and ELISA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 77411 |
|---|---|
| Other Names | 77411 |
| Purity | Anti-Esrp-2 is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-2 protein. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | Esrp2 |
|---|---|
| Synonyms | Rbm35b |
| Function | mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (By similarity). |
| Cellular Location | Nucleus. |
| Tissue Location | Epithelial cell-specific. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
