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Anti-Human RAD 54 (RABBIT) Antibody
RAD54 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, E, I, LCI |
| Application Note | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-25 of Human RAD54 protein. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 8438 |
|---|---|
| Other Names | 603761 |
| Purity | This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. BLAST analysis indicates 100 % homology of the immunizing sequence with RAD54 from human. Cross reactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | RAD54L |
|---|---|
| Synonyms | RAD54A |
| Function | Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks (PubMed:11459989, PubMed:12205100, PubMed:24798879, PubMed:27264870, PubMed:32457312, PubMed:9774452). Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (By similarity). |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination.
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