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Anti-Histone H3 [Trimethyl Lys9, p Thr6] (RABBIT) Antibody
Histone H3 K9me3/phospho T6 Antibody
![- Anti-Histone H3 [Trimethyl Lys9, p Thr6] (RABBIT) Antibody ASR5626](/assets/uploads/products/202407/ASR5626-HistoneH3-K9me3pT6-Antibody-1-IF-4x3.jpg)
- SPECIFICATION
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- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, IHC, I, LCI |
| Application Note | Anti-Histone H3 [Trimethyl Lys9, p Thr6] antibody is tested for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Histone H3 [Trimethyl Lys9, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated/phosphorylated peptide surrounding Lysine 9/Threonine 6 of human Histone H3.2. |
| Stabilizer | 30% Glycerol |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 126961;333932;653604 |
|---|---|
| Other Names | 126961 |
| Purity | Anti-Histone H3 [Trimethyl Lys9, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | H3C15 (HGNC:20505) |
|---|---|
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Cellular Location | Nucleus. Chromosome. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
H3K9 methylations are a conserved hallmark of heterochromatin binding domains. The trimethyl K9 version of histone H3, when coupled with a T6 phosphorylation, is related to the Oct4 and Nanog proteins in embryoid bodies. This association seems to indicate a role for this modified histone in cellular differentiation in development. The formation of this modification seems to be a result of a complicated multiple silencing process for downstream genes, which is RNAi independent. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
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