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Anti-Histone H3 [Trimethyl Lys4/ac Lys9] (RABBIT) Antibody
Histone H3 K4-Me3/K9-Ac Antibody
![- Anti-Histone H3 [Trimethyl Lys4/ac Lys9] (RABBIT) Antibody ASR5739](/assets/uploads/products/202407/ASR5739-HistoneH3-TrimethylLys4acLys9-1-IF-4x3.jpg)
- SPECIFICATION
- CITATIONS
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- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human, Mouse |
| Clonality | Polyclonal |
Application 
| WB, I, LCI |
| Application Note | Anti-Histone H3 K4-Me3/K9-Ac antibody is tested for dot blot, IF, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Histone H3 K4-Me3/K9-Ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a methylation surrounding Lysine 4 and an acetylation surrounding Lysine 9 of human Histone H3. |
| Stabilizer | 30% Glycerol |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 126961;333932;653604 |
|---|---|
| Purity | Anti-Histone H3 [Trimethyl Lys4/ac Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 K4-Me3/K9-Ac from other sources has not been determined. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | H3C15 (HGNC:20505) |
|---|---|
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Cellular Location | Nucleus. Chromosome. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Histones of the nucleosome build chromatin and undergo various post-translational modifications proven to regulate chromatin condensation and DNA accessibility. Histone H3 methylations on Lysine 4 is modified by Set7/9 enzyme and is associated with transcriptional activation. Acetylated Lysine 9 has been studied in regards to transcriptional activation and histone deposition. Anti-Histone H3 K4-Me3/K9-Ac antibody is ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
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