Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Anti-Histone H3 [ac Lys9/phospho Ser10] (RABBIT) Antibody
Histone H3 K9-Ac/phospho S10 (RABBIT) Antibody
![- Anti-Histone H3 [ac Lys9/phospho Ser10] (RABBIT) Antibody ASR5768](/assets/uploads/products/202407/ASR5768-Anti-Histone-H3-K9-AcpS10_1-IF-4x3.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human, Mouse |
| Clonality | Polyclonal |
Application 
| WB, I, LCI |
| Application Note | Anti-Histone H3 K9-Ac/pS10 antibody is tested for Western Blot, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15 kDa corresponding to Histone H3 protein by Western Blotting in NIH-3T3 histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Histone H3 [ac Lys9/phospho Ser10] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a acetylation surrounding Lysine 9 and a phosphorylation surrounding serine 10 of human Histone H3. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 126961;333932;653604 |
|---|---|
| Other Names | 126961 |
| Purity | Anti-Histone H3 K9Ac/pS10 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. Cross-reactivity with other sources has not been determined. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | H3C15 (HGNC:20505) |
|---|---|
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Cellular Location | Nucleus. Chromosome. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Histones of the nucleosome build chromatin and undergo various post-translational modifications proven to regulate chromatin condensation and DNA accessibility. Phosphorylations on Serine 10 has been shown to facilitate chromatin condensation by its interaction with Aurora-B kinase during mitosis and is alternatively active in cell division. Serine modification is associated with IKK-alpha and Snf1 enzymes in transcriptional activation, and is modified by MSK1 and 2 which are enzymes that react to growth factors and cellular stress in immediate-early gene activation. Lysine 9 acetylations are connected with histone deposition and transcriptional activation. Anti-Histone H3 [ac Lys9/phospho Ser10] antibody is ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
