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Anti-CATALASE (Bovine Liver) (RABBIT) Antibody (BULK ORDER)

Catalase Antibody

     
  •  - Anti-CATALASE (Bovine Liver) (RABBIT) Antibody (BULK ORDER) ASR5934
    Immunohistochemistry of Catalase antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Catalase antibody 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Liver parenchyma: portal triads affected by moderate lymphoplasmacytic inflammation with minimal proliferation of bile ductules. Not obvious interstitial fibrosis. Cytoplasma of hepatocytes, diffuse positivity for the antibody anti-catalase. Neg Ctr (far right) normal rabbit IgG with Human liver. pH 6.2 40X
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Product Information
Host Rabbit
Conjugate Unconjugated
Target Species Bovine
Reactivity Human
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, I, LCI
Application Note Anti-Catalase has been tested by ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Physical State Lyophilized
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.
Reconstitution Volume 5.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Gene ID 531682
Other Names 531682
Purity Anti-Catalase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine liver].  Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Name CAT
Function Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed:10691967). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed:10691967).
Cellular Location Peroxisome matrix.
Research Areas
Citations (0)
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Background

Anti-Catalase Antibody detects catalase. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. Catalase is necessary in cells to protect them from the toxic effects of hydrogen peroxide and to promote growth of cells. Anti-Catalase antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

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$ 0.00
Cat# ASR5934
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