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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ABHD5 Antibody (monoclonal) (M01)   

ABHD5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant ABHD5.

  • WB - ABHD5 Antibody (monoclonal) (M01) AT1012a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (64.13 KDa) .
  • WB - ABHD5 Antibody (monoclonal) (M01) AT1012a
    ABHD5 monoclonal antibody (M01), clone 1F3. Western Blot analysis of ABHD5 expression in rat testis.
  • WB - ABHD5 Antibody (monoclonal) (M01) AT1012a
    ABHD5 monoclonal antibody (M01), clone 1F3. Western Blot analysis of ABHD5 expression in HeLa.
  • WB - ABHD5 Antibody (monoclonal) (M01) AT1012a
    Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody (M01), clone 1F3.

    Lane 1: ABHD5 transfected lysate(39 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - ABHD5 Antibody (monoclonal) (M01) AT1012a
    Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
  • E - ABHD5 Antibody (monoclonal) (M01) AT1012a
    Detection limit for recombinant GST tagged ABHD5 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WTS1
Other Accession BC021958
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1F3
Calculated MW 39096 Da
Additional Information
Gene ID 51099
Other Names 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, Lipid droplet-binding protein CGI-58, ABHD5, NCIE2
Target/Specificity ABHD5 (AAH21958, 1 a.a. ~ 349 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsABHD5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.


1.Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.Patel S, Yang W, Kozusko K, Saudek V, Savage DBProc Natl Acad Sci U S A. 2014 Jun 24;111(25):9163-8. doi: 10.1073/pnas.1318791111. Epub 2014 Jun 9.2.microRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes.Kurtz CL, Peck BC, Fannin EE, Beysen C, Miao J, Landstreet SR, Ding S, Turaga V, Lund PK, Turner S, Biddinger SB, Vickers KC, Sethupathy PDiabetes. 2014 Apr 10.3.Endurance Exercise Training Up-Regulates Lipolytic Proteins and Reduces Triglyceride Content in Skeletal Muscle of Obese Subjects.Louche K, Badin PM, Montastier E, Laurens C, Bourlier V, de Glisezinski I, Thalamas C, Viguerie N, Langin D, Moro CJ Clin Endocrinol Metab. 2013 Oct 31.4.Downregulation of adipose triglyceride lipase in the heart aggravates diabetic cardiomyopathy in db/db mice.Inoue T, Kobayashi K, Inoguchi T, Sonoda N, Maeda Y, Hirata E, Fujimura Y, Miura D, Hirano K, Takayanagi RBiochem Biophys Res Commun. 2013 Aug 16;438(1):224-9. doi: 10.1016/j.bbrc.2013.07.063. Epub 2013 Jul 22.5.Deficiency of Liver Comparative Gene Identification-58 (CGI-58) Causes Steatohepatitis and Fibrosis in Mice.Guo F, Ma Y, Kadegowda AK, Xie P, Liu G, Liu X, Miao H, Ou J, Su X, Zheng Z, Xue B, Shi H, Yu LJ Lipid Res. 2013 Jun 3.6.Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier.Pollak NM, Schweiger M, Jaeger D, Kolb D, Kumari M, Schreiber R, Kolleritsch S, Markolin P, Grabner GF, Heier C, Zierler KA, Rulicke T, Zimmermann R, Lass A, Zechner R, Haemmerle GJ Lipid Res. 2013 Apr;54(4):1092-102. doi: 10.1194/jlr.M034710. Epub 2013 Jan 23.7.PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans.Grall A, Guaguere E, Planchais S, Grond S, Bourrat E, Hausser I, Hitte C, Le Gallo M, Derbois C, Kim GJ, Lagoutte L, Degorce-Rubiales F, Radner FP, Thomas A, Kury S, Bensignor E, Fontaine J, Pin D, Zimmermann R, Zechner R, Lathrop M, Galibert F, Andre C, Fischer J.Nat Genet. 2012 Jan 15;44(2):140-7. doi: 10.1038/ng.1056.8.Liver X Receptor (LXR) Regulates Human Adipocyte Lipolysis.Stenson BM, Ryden M, Venteclef N, Dahlman I, Pettersson AM, Mairal A, Astrom G, Blomqvist L, Wang V, Jocken JW, Clement K, Langin D, Arner P, Laurencikiene J.J Biol Chem. 2011 Jan 7;286(1):370-9. Epub 2010 Oct 28.9.B56{alpha}/Protein Phosphatase 2A Inhibits Adipose Lipolysis in High-Fat Diet-Induced Obese Mice.Kinney BP, Qiao L, Levaugh JM, Shao J.Endocrinology. 2010 Jun 9. [Epub ahead of print]

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$ 350.00
Cat# AT1012a
Availability: 7-10 days
Bulk Size

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