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ATP7B Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ATP7B.

     
  • WB - ATP7B Antibody (monoclonal) (M01) AT1244a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.08 KDa) .
    detail
  • WB - ATP7B Antibody (monoclonal) (M01) AT1244a
    ATP7B monoclonal antibody (M01), clone 3E10. Western Blot analysis of ATP7B expression in human colon.
    detail
  • E - ATP7B Antibody (monoclonal) (M01) AT1244a
    Detection limit for recombinant GST tagged ATP7B is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P35670
Other Accession NM_000053
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3E11
Calculated MW 157263 Da
Additional Information
Gene ID 540
Other Names Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein, WND/140 kDa, ATP7B, PWD, WC1, WND
Target/Specificity ATP7B (NP_000044, 1372 a.a. ~ 1465 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsATP7B Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).

References

1.Characterization of Sandwich-Cultured Hepatocytes as an In Vitro Model to Assess the Hepatobiliary Disposition of Copper.Ansede JH, Wright MR, St Claire RL, Hart RW, Gefroh HA, Brouwer KR.Drug Metab Dispos. 2009 May;37(5):969-76. Epub 2009 Feb 23.

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$ 350.00
Cat# AT1244a
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