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BLMH Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a partial recombinant BLMH.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q13867 |
| Other Accession | NM_000386 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 4A2 |
| Calculated MW | 52562 Da |
| Gene ID | 642 |
|---|---|
| Other Names | Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH |
| Target/Specificity | BLMH (NP_000377, 356 a.a. ~ 454 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | BLMH Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.
References
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Variation in bleomycin hydrolase gene is associated with reduced survival after chemotherapy for testicular germ cell cancer. de Haas EC, et al. J Clin Oncol, 2008 Apr 10. PMID 18398146.Micronuclei frequency induced by bleomycin in human peripheral lymphocytes: correlating BLHX polymorphism with mutagen sensitivity. Maffei F, et al. Mutat Res, 2008 Mar 1. PMID 18082847.Meta-analysis of genetic variability in the beta-amyloid production, aggregation and degradation metabolic pathways and the risk of Alzheimer's disease. Llorca J, et al. Acta Neurol Scand, 2008 Jan. PMID 17854420.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
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