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CD177 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CD177.

     
  • IP - CD177 Antibody (monoclonal) (M01) AT1431a
    Immunoprecipitation of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CD177 MaxPab rabbit polyclonal antibody.
    detail
  • WB - CD177 Antibody (monoclonal) (M01) AT1431a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - CD177 Antibody (monoclonal) (M01) AT1431a
    Western Blot analysis of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody (M01), clone 4C4.

    Lane 1: CD177 transfected lysate(46.4 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - CD177 Antibody (monoclonal) (M01) AT1431a
    Immunoperoxidase of monoclonal antibody to CD177 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]
    detail
  • E - CD177 Antibody (monoclonal) (M01) AT1431a
    Detection limit for recombinant GST tagged CD177 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, E
Primary Accession Q8N6Q3
Other Accession BC029167
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4C4
Calculated MW 46363 Da
Additional Information
Gene ID 57126
Other Names CD177 antigen, Human neutrophil alloantigen 2a, HNA-2a, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, CD177, CD177, NB1, PRV1
Target/Specificity CD177 (AAH29167, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCD177 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).

References

1.Gene expression analysis of a Helicobacter pylori-infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer.Toyoda T, Tsukamoto T, Yamamoto M, Ban H, Saito N, Takasu S, Shi L, Saito A, Ito S, Yamamura Y, Nishikawa A, Ogawa K, Tanaka T, Tatematsu MBMC Gastroenterol. 2013 Jul 30;13(1):122.

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$ 350.00
Cat# AT1431a
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