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CRSP7 Antibody (monoclonal) (M06)
Mouse monoclonal antibody raised against a partial recombinant CRSP7.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | O95402 |
| Other Accession | NM_004831 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 2G10 |
| Calculated MW | 65446 Da |
| Gene ID | 9441 |
|---|---|
| Other Names | Mediator of RNA polymerase II transcription subunit 26, Activator-recruited cofactor 70 kDa component, ARC70, Cofactor required for Sp1 transcriptional activation subunit 7, CRSP complex subunit 7, Mediator complex subunit 26, Transcriptional coactivator CRSP70, MED26, ARC70, CRSP7 |
| Target/Specificity | CRSP7 (NP_004822, 501 a.a. ~ 600 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | CRSP7 Antibody (monoclonal) (M06) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
References
MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression. Ding N, et al. J Biol Chem, 2009 Jan 30. PMID 19049968.Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. Zhang X, et al. Mol Cell, 2005 Jul 1. PMID 15989967.A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Sato S, et al. Mol Cell, 2004 Jun 4. PMID 15175163.Ras induces mediator complex exchange on C/EBP beta. Mo X, et al. Mol Cell, 2004 Jan 30. PMID 14759369.
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