DCTN2 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant DCTN2.
|Application ||WB, IF, E|
|Calculated MW||44231 Da|
|Other Names||Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, DCTN-50, p50 dynamitin, DCTN2, DCTN50|
|Target/Specificity||DCTN2 (AAH00718, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||DCTN2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.
Molecular and functional basis for the scaffolding role of the p50/dynamitin subunit of the microtubule-associated dynactin complex. Jacquot G, et al. J Biol Chem, 2010 Jul 23. PMID 20463029.Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes. Pridgeon JW, et al. FEBS J, 2009 Jan. PMID 19019082.N-terminal region of ZW10 serves not only as a determinant for localization but also as a link with dynein function. Inoue M, et al. Genes Cells, 2008 Aug. PMID 18782227.Dynamitin mutagenesis reveals protein-protein interactions important for dynactin structure. Maier KC, et al. Traffic, 2008 Apr. PMID 18182012.GSK-3beta-regulated interaction of BICD with dynein is involved in microtubule anchorage at centrosome. Fumoto K, et al. EMBO J, 2006 Dec 13. PMID 17139249.
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