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DHX8 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant DHX8.

     
  • WB - DHX8 Antibody (monoclonal) (M01) AT1760a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - DHX8 Antibody (monoclonal) (M01) AT1760a
    DHX8 monoclonal antibody (M01), clone 1E10 Western Blot analysis of DHX8 expression in Hela S3 NE ( (Cat # AT1760a )
    detail
  • E - DHX8 Antibody (monoclonal) (M01) AT1760a
    Detection limit for recombinant GST tagged DHX8 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q14562
Other Accession NM_004941
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1E11
Calculated MW 139315 Da
Additional Information
Gene ID 1659
Other Names ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1, DHX8, DDX8
Target/Specificity DHX8 (NP_004932, 301 a.a. ~ 400 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDHX8 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

References

Crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of the human spliceosomal DExD/H-box protein hPrp22. Kudlinzki D, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2009 Sep 1. PMID 19724143.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Novel function of beta-arrestin2 in the nucleus of mature spermatozoa. Neuhaus EM, et al. J Cell Sci, 2006 Aug 1. PMID 16820410.Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry. Tao WA, et al. Nat Methods, 2005 Aug. PMID 16094384.Nucleolar proteome dynamics. Andersen JS, et al. Nature, 2005 Jan 6. PMID 15635413.

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$ 350.00
Cat# AT1760a
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