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>   home   >   Products   >   Primary Antibodies   >   EEF1G Antibody (monoclonal) (M01)   

EEF1G Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant EEF1G.

     
  • IF - EEF1G Antibody (monoclonal) (M01) AT1857a
    Immunofluorescence of monoclonal antibody to EEF1G on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (73.59 KDa) .
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    EEF1G monoclonal antibody (M01), clone 3F11-1A10. Western Blot analysis of EEF1G expression in PC-12((Cat # AT1857a )
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    EEF1G monoclonal antibody (M01), clone 3F11-1A10. Western Blot analysis of EEF1G expression in HepG2 ( (Cat # AT1857a )
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    EEF1G monoclonal antibody (M01), clone 3F11-1A10. Western Blot analysis of EEF1G expression in 293 ( (Cat # AT1857a )
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    EEF1G monoclonal antibody (M01), clone 3F11-1A10 Western Blot analysis of EEF1G expression in HL-60 ( (Cat # AT1857a )
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    EEF1G monoclonal antibody (M01), clone 3F11-1A10. Western Blot analysis of EEF1G expression in Raw 264.7((Cat # AT1857a )
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    EEF1G monoclonal antibody (M01), clone 3F11-1A10. Western Blot analysis of EEF1G expression in NIH/3T3((Cat # AT1857a )
    detail
  • WB - EEF1G Antibody (monoclonal) (M01) AT1857a
    Western Blot analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.

    Lane 1: EEF1G transfected lysate(50 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - EEF1G Antibody (monoclonal) (M01) AT1857a
    Detection limit for recombinant GST tagged EEF1G is approximately 0.1ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P26641
Other Accession BC015813
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3F11-1A10
Calculated MW 50119 Da
Additional Information
Gene ID 1937
Other Names Elongation factor 1-gamma, EF-1-gamma, eEF-1B gamma, EEF1G, EF1G
Target/Specificity EEF1G (AAH15813.1, 1 a.a. ~ 437 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsEEF1G Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

References

1.The eEF1 gamma Subunit Contacts RNA Polymerase II and Binds Vimentin Promoter Region.Corbi N, Batassa EM, Pisani C, Onori A, Di Certo MG, Strimpakos G, Fanciulli M, Mattei E, Passananti C.PLoS One. 2010 Dec 31;5(12):e14481.

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$ 350.00
Cat# AT1857a
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