FKSG14 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant CENPK.
|Application ||WB, IF, E|
|Calculated MW||31655 Da|
|Other Names||Centromere protein K, CENP-K, Interphase centromere complex protein 37, Protein AF-5alpha, p33, CENPK, ICEN37|
|Target/Specificity||CENPK (AAH05400.1, 1 a.a. ~ 269 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||FKSG14 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).
KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates. Cheeseman IM, et al. Mol Biol Cell, 2008 Feb. PMID 18045986.Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells. Izuta H, et al. Genes Cells, 2006 Jun. PMID 16716197.The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Okada M, et al. Nat Cell Biol, 2006 May. PMID 16622420.The human CENP-A centromeric nucleosome-associated complex. Foltz DR, et al. Nat Cell Biol, 2006 May. PMID 16622419.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
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