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G3BP Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant G3BP.

     
  • IF - G3BP Antibody (monoclonal) (M01) AT2137a
    Immunofluorescence of monoclonal antibody to G3BP on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - G3BP Antibody (monoclonal) (M01) AT2137a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.53 KDa) .
    detail
  • WB - G3BP Antibody (monoclonal) (M01) AT2137a
    G3BP monoclonal antibody (M01), clone 2F3 Western Blot analysis of G3BP expression in A-431 ( (Cat # AT2137a )
    detail
  • WB - G3BP Antibody (monoclonal) (M01) AT2137a
    Western Blot analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody (M01), clone 2F3.

    Lane 1: G3BP transfected lysate(52.2 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - G3BP Antibody (monoclonal) (M01) AT2137a
    Immunoperoxidase of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 1 ug/ml]
    detail
  • E - G3BP Antibody (monoclonal) (M01) AT2137a
    Detection limit for recombinant GST tagged G3BP is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q13283
Other Accession BC006997
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2F3
Calculated MW 52164 Da
Additional Information
Gene ID 10146
Other Names Ras GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP
Target/Specificity G3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsG3BP Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

References

1.5-Fluorouracil affects assembly of stress granules based on RNA incorporation.Kaehler C, Isensee J, Hucho T, Lehrach H, Krobitsch SNucleic Acids Res. 2014 Apr 11.2.Deficiency of G3BP1, the stress granules assembly factor, results in abnormal synaptic plasticity and calcium homeostasis in neurons.Martin S, Zekri L, Metz A, Maurice T, Chebli K, Vignes M, Tazi JJ Neurochem. 2013 Feb 4. doi: 10.1111/jnc.12189.3.Progressive loss of dopaminergic neurons induced by unilateral rotenone infusion into the medial forebrain bundle.Norazit A, Meedeniya AC, Nguyen MN, Mackay-Sim A.Brain Res. 2010 Aug 28. [Epub ahead of print]

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$ 350.00
Cat# AT2137a
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