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HIF1AN Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full-length recombinant HIF1AN.

  • IF - HIF1AN Antibody (monoclonal) (M01) AT2369a
    Immunofluorescence of monoclonal antibody to HIF1AN on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - HIF1AN Antibody (monoclonal) (M01) AT2369a
    Immunoprecipitation of HIF1AN transfected lysate using anti-HIF1AN monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HIF1AN monoclonal antibody.
  • WB - HIF1AN Antibody (monoclonal) (M01) AT2369a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (64.13 KDa) .
  • WB - HIF1AN Antibody (monoclonal) (M01) AT2369a
    HIF1AN monoclonal antibody (M01), clone 1D8 Western Blot analysis of HIF1AN expression in Jurkat ( (Cat # AT2369a )
  • WB - HIF1AN Antibody (monoclonal) (M01) AT2369a
    Western Blot analysis of HIF1AN expression in transfected 293T cell line by HIF1AN monoclonal antibody (M01), clone 1D8.

    Lane 1: HIF1AN transfected lysate (Predicted MW: 40.3 KDa).
    Lane 2: Non-transfected lysate.
  • E - HIF1AN Antibody (monoclonal) (M01) AT2369a
    Detection limit for recombinant GST tagged HIF1AN is 0.1 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NWT6
Other Accession BC007719
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1D8
Calculated MW 40285 Da
Additional Information
Gene ID 55662
Other Names Hypoxia-inducible factor 1-alpha inhibitor, 11411n4, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase, HIF1AN, FIH1
Target/Specificity HIF1AN (AAH07719.1, 1 a.a. ~ 349 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHIF1AN Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.miR-31 ablates expression of the HIF regulatory factor FIH to activate the HIF pathway in head and neck carcinoma. Liu CJ, et al. Cancer Res, 2010 Feb 15. PMID 20145132.Genome-Wide Scan of Copy Number Variation in Late-Onset Alzheimer's Disease. Heinzen EL, et al. J Alzheimers Dis, 2009 Sep 11. PMID 19749422.Mint3 enhances the activity of hypoxia-inducible factor-1 (HIF-1) in macrophages by suppressing the activity of factor inhibiting HIF-1. Sakamoto T, et al. J Biol Chem, 2009 Oct 30. PMID 19726677.Nutlin-3, an Hdm2 antagonist, inhibits tumor adaptation to hypoxia by stimulating the FIH-mediated inactivation of HIF-1alpha. Lee YM, et al. Carcinogenesis, 2009 Oct. PMID 19696166.

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$ 350.00
Cat# AT2369a
Availability: 7-10 days
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