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HMGB1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant HMGB1.

  • IF - HMGB1 Antibody (monoclonal) (M02) AT2384a
    Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml]
  • WB - HMGB1 Antibody (monoclonal) (M02) AT2384a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (49.39 KDa) .
  • WB - HMGB1 Antibody (monoclonal) (M02) AT2384a
    HMGB1 monoclonal antibody (M02), clone 1D5 Western Blot analysis of HMGB1 expression in K-562 ( (Cat # AT2384a )
  • WB - HMGB1 Antibody (monoclonal) (M02) AT2384a
    Western Blot analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M02), clone 1D5.

    Lane 1: HMGB1 transfected lysate(24.9 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - HMGB1 Antibody (monoclonal) (M02) AT2384a
    Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09429
Other Accession BC003378
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1D5
Calculated MW 24894 Da
Additional Information
Gene ID 3146
Other Names High mobility group protein B1, High mobility group protein 1, HMG-1, HMGB1, HMG1
Target/Specificity HMGB1 (AAH03378.1, 1 a.a. ~ 215 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHMGB1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1.TLR4, IL-6, IL-18, MyD88 and HMGB1 are highly expressed in intracranial inflammatory lesions and the IgG4/IgG ratio correlates with TLR4 and IL-6.Hirano H, Yoshioka T, Yunoue S, Fujio S, Yonezawa H, Niiro T, Habu M, Oyoshi T, Sugata S, Kamezawa T, Arimura H, Hanaya R, Tokimura H, Tokudome M, Arita K.Neuropathology. 2012 Mar 13. doi: 10.1111/j.1440-1789.2012.01310.x. [Epub ahead of print]2.Human IgG antibody profiles differentiate between symptomatic patients with and without colorectal cancer.Kijanka G, Hector S, Kay EW, Murray F, Cummins R, Murphy D, Maccraith BD, Prehn JH, Kenny D.Gut. 2010 Jan;59(1):69-78.3.Donor Toll-like receptor 4 contributes to ischemia and reperfusion injury following human kidney transplantation.Kruger B, Krick S, Dhillon N, Lerner SM, Ames S, Bromberg JS, Lin M, Walsh L, Vella J, Fischereder M, Kramer BK, Colvin RB, Heeger PS, Murphy BT, Schroppel B.Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3390-5. Epub 2009 Feb 13.

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$ 350.00
Cat# AT2384a
Availability: 7-10 days
Bulk Size

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