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ID3 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant ID3.

     
  • IF - ID3 Antibody (monoclonal) (M02) AT2477a
    Immunofluorescence of monoclonal antibody to ID3 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - ID3 Antibody (monoclonal) (M02) AT2477a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (34.87 KDa) .
    detail
  • E - ID3 Antibody (monoclonal) (M02) AT2477a
    Detection limit for recombinant GST tagged ID3 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q02535
Other Accession NM_002167
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG3 Kappa
Clone Names 3E11
Calculated MW 12999 Da
Additional Information
Gene ID 3399
Other Names DNA-binding protein inhibitor ID-3, Class B basic helix-loop-helix protein 25, bHLHb25, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, Inhibitor of differentiation 3, ID3, 1R21, BHLHB25, HEIR1
Target/Specificity ID3 (NP_002158, 1 a.a. ~ 83 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsID3 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

References

Id3 is a novel atheroprotective factor containing a functionally significant single-nucleotide polymorphism associated with intima-media thickness in humans. Doran AC, et al. Circ Res, 2010 Apr 16. PMID 20185798.Integrative predictive model of coronary artery calcification in atherosclerosis. McGeachie M, et al. Circulation, 2009 Dec 15. PMID 19948975.Id1/Id3 knockdown inhibits metastatic potential of pancreatic cancer. Shuno Y, et al. J Surg Res, 2010 Jun 1. PMID 19515385.Fibroblast growth factor 2 reactivates G1 checkpoint in SK-N-MC cells via regulation of p21, inhibitor of differentiation genes (Id1-3), and epithelium-mesenchyme transition-like events. Higgins S, et al. Endocrinology, 2009 Sep. PMID 19477940.UVB upregulates the bax promoter in immortalized human keratinocytes via ROS induction of Id3. Trabosh VA, et al. Exp Dermatol, 2009 Apr. PMID 19054058.

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$ 350.00
Cat# AT2477a
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