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IRX5 Antibody (monoclonal) (M06)

Mouse monoclonal antibody raised against a partial recombinant IRX5.

  • IF - IRX5 Antibody (monoclonal) (M06) AT2555a
    Immunofluorescence of monoclonal antibody to IRX5 on HeLa cell. [antibody concentration 10 ug/ml]
  • WB - IRX5 Antibody (monoclonal) (M06) AT2555a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (32.45 KDa) .
  • WB - IRX5 Antibody (monoclonal) (M06) AT2555a
    IRX5 monoclonal antibody (M06), clone 8A12. Western Blot analysis of IRX5 expression in Raw 264.7.
  • WB - IRX5 Antibody (monoclonal) (M06) AT2555a
    IRX5 monoclonal antibody (M06), clone 8A12. Western Blot analysis of IRX5 expression in NIH/3T3((Cat # AT2555a )
  • E - IRX5 Antibody (monoclonal) (M06) AT2555a
    Detection limit for recombinant GST tagged IRX5 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P78411
Other Accession NM_005853
Reactivity Human, Mouse
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 8A12
Calculated MW 50361 Da
Additional Information
Gene ID 10265
Other Names Iroquois-class homeodomain protein IRX-5, Homeodomain protein IRX-2A, Homeodomain protein IRXB2, Iroquois homeobox protein 5, IRX5, IRX2A, IRXB2
Target/Specificity IRX5 (NP_005844, 204 a.a. ~ 264 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIRX5 Antibody (monoclonal) (M06) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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IRX5 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.


The iroquois homeobox gene 5 is regulated by 1,25-dihydroxyvitamin D3 in human prostate cancer and regulates apoptosis and the cell cycle in LNCaP prostate cancer cells. Myrthue A, et al. Clin Cancer Res, 2008 Jun 1. PMID 18519790.A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses. Kanno S, et al. EMBO J, 2007 Apr 18. PMID 17396150.[Irx5: a transcription factor that regulates the cardiac repolarization gradient] Bruneau BG. Med Sci (Paris), 2006 Mar. PMID 16527195.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.Cloning and chromosome mapping of human and chicken Iroquois (IRX) genes. Ogura K, et al. Cytogenet Cell Genet, 2001. PMID 11435706.

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$ 350.00
Cat# AT2555a
Availability: 7-10 days
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