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KCNJ15 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant KCNJ15.

     
  • WB - KCNJ15 Antibody (monoclonal) (M01) AT2599a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (33 KDa) .
    detail
  • WB - KCNJ15 Antibody (monoclonal) (M01) AT2599a
    Western Blot analysis of KCNJ15 expression in transfected 293T cell line by KCNJ15 monoclonal antibody (M01), clone 1B2.

    Lane 1: KCNJ15 transfected lysate(42.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - KCNJ15 Antibody (monoclonal) (M01) AT2599a
    Western blot analysis of KCNJ15 over-expressed 293 cell line, cotransfected with KCNJ15 Validated Chimera RNAi ( (Cat # AT2599a )
    detail
  • E - KCNJ15 Antibody (monoclonal) (M01) AT2599a
    Detection limit for recombinant GST tagged KCNJ15 is 3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q99712
Other Accession NM_002243
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1B2
Calculated MW 42577 Da
Additional Information
Gene ID 3772
Other Names ATP-sensitive inward rectifier potassium channel 15, Inward rectifier K(+) channel Kir13, Inward rectifier K(+) channel Kir42, Potassium channel, inwardly rectifying subfamily J member 15, KCNJ15, KCNJ14
Target/Specificity KCNJ15 (NP_002234, 290 a.a. ~ 355 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsKCNJ15 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Three transcript variants encoding the same protein have been found for this gene.

References

Identification of KCNJ15 as a susceptibility gene in Asian patients with type 2 diabetes mellitus. Okamoto K, et al. Am J Hum Genet, 2010 Jan. PMID 20085713.MUPP1 complexes renal K+ channels to alter cell surface expression and whole cell currents. Sindic A, et al. Am J Physiol Renal Physiol, 2009 Jul. PMID 19420109.Rare independent mutations in renal salt handling genes contribute to blood pressure variation. Ji W, et al. Nat Genet, 2008 May. PMID 18391953.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Interaction of the Ca2+-sensing receptor with the inwardly rectifying potassium channels Kir4.1 and Kir4.2 results in inhibition of channel function. Huang C, et al. Am J Physiol Renal Physiol, 2007 Mar. PMID 17122384.

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$ 350.00
Cat# AT2599a
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Availability: 7-10 days
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