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KNTC1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant KNTC1.

     
  • IF - KNTC1 Antibody (monoclonal) (M01) AT2645a
    Immunofluorescence of monoclonal antibody to KNTC1 on HeLa cell. [antibody concentration 50 ug/ml]
    detail
  • WB - KNTC1 Antibody (monoclonal) (M01) AT2645a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - KNTC1 Antibody (monoclonal) (M01) AT2645a
    KNTC1 monoclonal antibody (M01), clone 10H4 Western Blot analysis of KNTC1 expression in Hela S3 NE ( (Cat # AT2645a )
    detail
  • E - KNTC1 Antibody (monoclonal) (M01) AT2645a
    Detection limit for recombinant GST tagged KNTC1 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P50748
Other Accession NM_014708
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 10H4
Calculated MW 250749 Da
Additional Information
Gene ID 9735
Other Names Kinetochore-associated protein 1, Rough deal homolog, HsROD, Rod, hRod, KNTC1, KIAA0166
Target/Specificity KNTC1 (NP_055523, 2100 a.a. ~ 2209 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsKNTC1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein.

References

1.Modulations of cell cycle checkpoints during HCV associated disease.Sarfraz S, Hamid S, Ali S, Jafri W, Siddiqui AA.BMC Infect Dis. 2009 Aug 10;9:125.2.Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A.J Cell Biol. 2009 Jun 1;185(5):859-74. Epub 2009 May 25.

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$ 350.00
Cat# AT2645a
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Availability: 7-10 days
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