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MPHOSPH10 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant MPHOSPH10.

     
  • IF - MPHOSPH10 Antibody (monoclonal) (M02) AT2891a
    Immunofluorescence of monoclonal antibody to MPHOSPH10 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - MPHOSPH10 Antibody (monoclonal) (M02) AT2891a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - MPHOSPH10 Antibody (monoclonal) (M02) AT2891a
    MPHOSPH10 monoclonal antibody (M02), clone 1B10 Western Blot analysis of MPHOSPH10 expression in Hela S3 NE ( (Cat # AT2891a )
    detail
  • E - MPHOSPH10 Antibody (monoclonal) (M02) AT2891a
    Detection limit for recombinant GST tagged MPHOSPH10 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O00566
Other Accession NM_005791
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1B10
Calculated MW 78864 Da
Additional Information
Gene ID 10199
Other Names U3 small nucleolar ribonucleoprotein protein MPP10, M phase phosphoprotein 10, MPHOSPH10, MPP10
Target/Specificity MPHOSPH10 (NP_005782, 348 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMPHOSPH10 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
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Background

This gene encodes a protein that is phosphorylated during mitosis. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein is thought to be part of the U3 small nucleolar ribonucleoprotein complex, which is involved in rRNA processing.

References

Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.Nucleolar proteome dynamics. Andersen JS, et al. Nature, 2005 Jan 6. PMID 15635413.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.The human Imp3 and Imp4 proteins form a ternary complex with hMpp10, which only interacts with the U3 snoRNA in 60-80S ribonucleoprotein complexes. Granneman S, et al. Nucleic Acids Res, 2003 Apr 1. PMID 12655004.

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$ 350.00
Cat# AT2891a
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Availability: 7-10 days
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