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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MPP1 Antibody (monoclonal) (M01)   

MPP1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant MPP1.

  • IF - MPP1 Antibody (monoclonal) (M01) AT2893a
    Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell . [antibody concentration 10 ug/ml]
  • IP - MPP1 Antibody (monoclonal) (M01) AT2893a
    Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MPP1 monoclonal antibody.
  • WB - MPP1 Antibody (monoclonal) (M01) AT2893a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (77 KDa) .
  • WB - MPP1 Antibody (monoclonal) (M01) AT2893a
    MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.
  • WB - MPP1 Antibody (monoclonal) (M01) AT2893a
    Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.

    Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).
    Lane 2: Non-transfected lysate.
  • E - MPP1 Antibody (monoclonal) (M01) AT2893a
    Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q00013
Other Accession BC002392
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1E11-1G11
Calculated MW 52296 Da
Additional Information
Gene ID 4354
Other Names 55 kDa erythrocyte membrane protein, p55, Membrane protein, palmitoylated 1, MPP1, DXS552E, EMP55
Target/Specificity MPP1 (AAH02392, 1 a.a. ~ 466 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMPP1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.


Identification of erythrocyte p55/MPP1 as a binding partner of NF2 tumor suppressor protein/Merlin. Seo PS, et al. Exp Biol Med (Maywood), 2009 Mar. PMID 19144871.Alternatively spliced exon 5 of the FERM domain of protein 4.1R encodes a novel binding site for erythrocyte p55 and is critical for membrane targeting in epithelial cells. Seo PS, et al. Biochim Biophys Acta, 2009 Feb. PMID 18952129.MPP1 links the Usher protein network and the Crumbs protein complex in the retina. Gosens I, et al. Hum Mol Genet, 2007 Aug 15. PMID 17584769.Solution structure of human erythroid p55 PDZ domain. Kusunoki H, et al. Proteins, 2006 Aug 15. PMID 16741958.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.

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$ 350.00
Cat# AT2893a
Availability: 7-10 days
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