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NEK11 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant NEK11.

  • IF - NEK11 Antibody (monoclonal) (M01) AT3014a
    Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10 ug/ml]
  • WB - NEK11 Antibody (monoclonal) (M01) AT3014a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (78.54 KDa) .
  • WB - NEK11 Antibody (monoclonal) (M01) AT3014a
    NEK11 monoclonal antibody (M01), clone 4E1-1F4 Western Blot analysis of NEK11 expression in HL-60 ( (Cat # AT3014a )
  • WB - NEK11 Antibody (monoclonal) (M01) AT3014a
    NEK11 monoclonal antibody (M01), clone 4E1-1F4. Western Blot analysis of NEK11 expression in A-431 ( (Cat # AT3014a )
  • WB - NEK11 Antibody (monoclonal) (M01) AT3014a
    Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody (M01), clone 4E1-1F4.

    Lane 1: NEK11 transfected lysate(55.5 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - NEK11 Antibody (monoclonal) (M01) AT3014a
    Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3 ug/ml]
  • E - NEK11 Antibody (monoclonal) (M01) AT3014a
    Detection limit for recombinant GST tagged NEK11 is 0.1 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NG66
Other Accession BC028587
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 4E1-1F4
Calculated MW 74192 Da
Additional Information
Gene ID 79858
Other Names Serine/threonine-protein kinase Nek11, Never in mitosis A-related kinase 11, NimA-related protein kinase 11, NEK11 (HGNC:18593)
Target/Specificity NEK11 (AAH28587, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNEK11 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling. S?rensen CS, et al. Cell Cycle, 2010 Feb 1. PMID 20090422.NEK11 regulates CDC25A degradation and the IR-induced G2/M checkpoint. Melixetian M, et al. Nat Cell Biol, 2009 Oct. PMID 19734889.Molecular genetics of successful smoking cessation: convergent genome-wide association study results. Uhl GR, et al. Arch Gen Psychiatry, 2008 Jun. PMID 18519826.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.

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$ 350.00
Cat# AT3014a
Availability: 7-10 days
Bulk Size

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