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NMI Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NMI.

     
  • WB - NMI Antibody (monoclonal) (M01) AT3070a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
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  • WB - NMI Antibody (monoclonal) (M01) AT3070a
    NMI monoclonal antibody (M01), clone 9D8 Western Blot analysis of NMI expression in HeLa ( (Cat # AT3070a )
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  • WB - NMI Antibody (monoclonal) (M01) AT3070a
    Western Blot analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody (M01), clone 9D8.

    Lane 1: NMI transfected lysate(35.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - NMI Antibody (monoclonal) (M01) AT3070a
    Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi ( (Cat # AT3070a )
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  • IHC - NMI Antibody (monoclonal) (M01) AT3070a
    Immunoperoxidase of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
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  • E - NMI Antibody (monoclonal) (M01) AT3070a
    Detection limit for recombinant GST tagged NMI is approximately 0.03ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q13287
Other Accession BC001268
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 9D8
Calculated MW 35057 Da
Additional Information
Gene ID 9111
Other Names N-myc-interactor, Nmi, N-myc and STAT interactor, NMI
Target/Specificity NMI (AAH01268, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNMI Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

References

New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.Nmi (N-Myc interactor) inhibits Wnt/beta-catenin signaling and retards tumor growth. Fillmore RA, et al. Int J Cancer, 2009 Aug 1. PMID 19358268.Tagging single-nucleotide polymorphisms in candidate oncogenes and susceptibility to ovarian cancer. Quaye L, et al. Br J Cancer, 2009 Mar 24. PMID 19240718.Evaluating new candidate SNPs as low penetrance risk factors in sporadic breast cancer: a two-stage Spanish case-control study. Vega A, et al. Gynecol Oncol, 2009 Jan. PMID 18950845.The effects of common genetic variants in oncogenes on ovarian cancer survival. Quaye L, et al. Clin Cancer Res, 2008 Sep 15. PMID 18794094.

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$ 350.00
Cat# AT3070a
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Availability: 7-10 days
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