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RRM2 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RRM2.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, IP, E |
|---|---|
| Primary Accession | P31350 |
| Other Accession | NM_001034 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 10 |
| Calculated MW | 44878 Da |
| Gene ID | 6241 |
|---|---|
| Other Names | Ribonucleoside-diphosphate reductase subunit M2, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit, RRM2, RR2 |
| Target/Specificity | RRM2 (NP_001025, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 IP~~N/A E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RRM2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.
References
1.RRM1 maintains centrosomal integrity via CHK1 and CDK1 signaling during replication stress.Kim SH, Park ER, Joo HY, Shen YN, Hong SH, Kim CH, Singh R, Lee KH, Shin HJCancer Lett. 2014 Jan 14. pii: S0304-3835(14)00014-7. doi: 10.1016/j.canlet.2013.12.031.2.KRAS-mediated Up-regulation of RRM2 Expression Is Essential for the Proliferation of Colorectal Cancer Cell Lines.Yoshida Y, Tsunoda T, Doi K, Tanaka Y, Fujimoto T, Machida T, Ota T, Koyanagi M, Takashima Y, Sasazuki T, Kuroki M, Iwasaki A, Shirasawa S.Anticancer Research July 2011 vol. 31 no. 7 2535-25393.Epstein-Barr virus episome stability is coupled to a delay in replication timing.Zhou J, Snyder A, Lieberman PM.J Virol. 2009 Mar;83(5):2154-62. Epub 2008 Dec 10.4.Quantitative phosphoproteome profiling of Wnt3a mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine-20 in canonical Wnt signal transduction.Tang LY, Deng N, Wang LS, Dai J, Wang ZL, Jiang XS, Li SJ, Li L, Sheng QH, Wu DQ, Li L, Zeng R.Mol Cell Proteomics. 2007 Nov;6(11):1952-67. Epub 2007 Aug 12.
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