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UBE2G2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant UBE2G2.

     
  • WB - UBE2G2 Antibody (monoclonal) (M01) AT4436a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.31 KDa) .
    detail
  • WB - UBE2G2 Antibody (monoclonal) (M01) AT4436a
    UBE2G2 monoclonal antibody (M01), clone 5E1 Western Blot analysis of UBE2G2 expression in HeLa ( (Cat # AT4436a )
    detail
  • WB - UBE2G2 Antibody (monoclonal) (M01) AT4436a
    Western Blot analysis of UBE2G2 expression in transfected 293T cell line by UBE2G2 monoclonal antibody (M01), clone 5E1.

    Lane 1: UBE2G2 transfected lysate(15.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - UBE2G2 Antibody (monoclonal) (M01) AT4436a
    Immunoperoxidase of monoclonal antibody to UBE2G2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 1 ug/ml]
    detail
  • E - UBE2G2 Antibody (monoclonal) (M01) AT4436a
    Detection limit for recombinant GST tagged UBE2G2 is approximately 0.1ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P60604
Other Accession NM_003343
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 50
Calculated MW 18566 Da
Additional Information
Gene ID 7327
Other Names Ubiquitin-conjugating enzyme E2 G2, Ubiquitin carrier protein G2, Ubiquitin-protein ligase G2, UBE2G2
Target/Specificity UBE2G2 (NP_003329, 1 a.a. ~ 87 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUBE2G2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

References

1.Ataxin-3 Deubiquitination Is Coupled to Parkin Ubiquitination via E2 Ubiquitin-conjugating Enzyme.Durcan TM, Kontogiannea M, Bedard N, Wing SS, Fon EA.J Biol Chem. 2012 Jan 2;287(1):531-41. Epub 2011 Nov 11.

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$ 350.00
Cat# AT4436a
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