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ZNF274 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ZNF274.

     
  • IF - ZNF274 Antibody (monoclonal) (M01) AT4620a
    Immunofluorescence of monoclonal antibody to ZNF274 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - ZNF274 Antibody (monoclonal) (M01) AT4620a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.95 KDa) .
    detail
  • WB - ZNF274 Antibody (monoclonal) (M01) AT4620a
    Western Blot analysis of ZNF274 expression in transfected 293T cell line by ZNF274 monoclonal antibody (M01), clone 4C12.

    Lane 1: ZNF274 transfected lysate(74.2 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - ZNF274 Antibody (monoclonal) (M01) AT4620a
    Detection limit for recombinant GST tagged ZNF274 is 0.1 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q96GC6
Other Accession NM_133502
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 4C12
Calculated MW 74177 Da
Additional Information
Gene ID 10782
Other Names Neurotrophin receptor-interacting factor homolog, Zinc finger protein 274, Zinc finger protein HFB101, Zinc finger protein with KRAB and SCAN domains 19, Zinc finger protein zfp2, Zf2, ZNF274, ZKSCAN19
Target/Specificity ZNF274 (NP_598009, 420 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsZNF274 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
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Background

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.

References

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. Wu C, et al. Proteomics, 2007 Jun. PMID 17474147.The KAP1 corepressor functions to coordinate the assembly of de novo HP1-demarcated microenvironments of heterochromatin required for KRAB zinc finger protein-mediated transcriptional repression. Sripathy SP, et al. Mol Cell Biol, 2006 Nov. PMID 16954381.Large-scale cDNA transfection screening for genes related to cancer development and progression. Wan D, et al. Proc Natl Acad Sci U S A, 2004 Nov 2. PMID 15498874.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.

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$ 350.00
Cat# AT4620a
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