Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
APC-Cy7 Anti-Mouse CD45.2 (104) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC |
|---|---|
| Isotype | Mouse IgG2a, kappa |
| Concentration | 0.2 mg/mL |
| Reactivity | Mouse |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
| Host | Mouse |
| Gene ID | 19264 |
|---|---|
| Alternative Name(s) | Ly5.2 |
| Format | APC-Cy7 |
| Preparation | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
| Application Notes | This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
| Storage Conditions | 2-8°C protected from light |
Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675. (Flow Cytometry)
Hale JS, Nelson LT, Simmons KB, and Fink PJ. 2011. J. Immunol. 186: 799 - 806. (Flow Cytometry)
Orr MT, Beilke JN, Proekt I, and Lanier LL. 2010. Proc. Natl. Acad. Sci. 107: 15844 - 15849. (Flow Cytometry)
Banerjee K, Biswas PS, Kumaraguru U, Schoenberger SP, and Rouse BT. 2004. 173: 7575-7583. (Immunofluorescence microscopy – frozen tissue)
Favre CJ, Mancuso M, Maas K, McLean JW, Baluk P, and Mcdonald DM. 2003. Am. J. Physiol. Heart Circ. Physiol. 285:H1917-H1938. (Immunocytochemistry)
Shen F-W, Tung J-S, and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation).
Provided below are standard protocols that you may find useful for product applications.
Background
The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
