In Vivo Ready™ Anti-Mouse CD28 (37.51) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IP, FA |
---|---|
Isotype | Golden Syrian Hamster IgG |
Concentration | 2 mg/mL |
Reactivity | Mouse |
Formulation | 10 mM NaH2PO4, 150 mM NaCl, pH7.2 |
Host | Golden Syrian Hamster |
Gene ID | 12487 |
---|---|
Gene Name | Cd28 |
Alternative Name(s) | Tp44, T44 |
Format | In Vivo Ready™ |
Preparation | This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze. |
Application Notes | This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Citations are provided as a convenience to you - please consult Materials and Methods sections for additional details about the use of any product in these publications. |
Endotoxin Level | Less than or equal to 0.01 EU/ug, as determined by the LaL assay |
Storage Conditions | 2-8°C |
Johnston RJ, Choi YS, Diamond JA, Yang JA, and Crotty S. 2012. J. Exp. Med. 209:243-250. (in vitro activation)
Hafalla JCR, Burgold J, Dorhoi A, Gross O, Ruland J, Kaufmann SHE, and Matuschewski K. 2012. Infect. Immun. 80:1274-1279. (in vitro activation)
Driessens G, Zheng Y, Locke F, Cannon JL, Gounari F, and Gajewski TF. 2011. J. Immunol. 186:784-790. (Flow cytometry)
Alcazar I, Cortes I, Zaballos A, Hernandez C, Fruman DA, Barber DF, and Carrera AC. 2009. Blood. 113:3198-3208. (Immunoprecipitation, in vitro activation
Albert MH, Yu X-Z, Martin PJ, and Anasetti C. 2005. Blood. 105:1355-1361. (in vivo activation)
Provided below are standard protocols that you may find useful for product applications.
Background
The 37.51 antibody reacts with mouse CD28, a 45 kDa glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of co-stimulatory receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.
The 37.51 may be used as a phenotypic marker for CD28, which is expressed on all CD4+ T cells and CD8+ T cells, and on NK cells in mouse. In addition, the 37.51 antibody is widely used to activate the CD28 receptor in vitro and in vivo.
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