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In Vivo Ready™ Anti-Mouse IL-4 (11B11) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-F, IF, FC, E, FA |
|---|---|
| Isotype | Rat IgG1, kappa |
| Concentration | 2 mg/mL |
| Reactivity | Mouse |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, pH7.2 |
| Host | Rat |
| Gene ID | 16189 |
|---|---|
| Gene Name | Il4 |
| Alternative Name(s) | IL4, Interleukin-4, BSF-1 , nale, na/le, leaf, ultra leaf, ultra-leaf, functional grade |
| Format | In Vivo Ready™ |
| Preparation | This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze. |
| Application Notes | This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Citations are provided as a convenience to you - please consult Materials and Methods sections for additional details about the use of any product in these publications. |
| Endotoxin Level | Less than or equal to 0.01 EU/ug, as determined by the LaL assay |
| Storage Conditions | 2-8°C |
Cook PC, Jones LH, Jenkins SJ, Wynn TA, Allen JE, and MacDonald AS. 2012. Proc. Natl. Acad. Sci. 109: 9977-9982. (in vivo blocking)
Altin JA, Goodnow CC, and Cook MC. 2012. J. Immunol. 5478-5488. (Flow cytometry)
Tofukuji S, Kuwahara M, Suzuki J, Ohara O, Nakayama T, and Yamashita M. 2012. J. Immunol. 188: 4846-4857. (in vitro Th1 polarization)
Weber KS, Hildner K, Murphy KM and Allen PM. 2010 J. Immunol. 185: 2836-2846 (in vitro Th1 polarization, ELISA)
Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy – frozen tissue, Immunohistochemistry – frozen tissue)
Provided below are standard protocols that you may find useful for product applications.
Background
The 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14 kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).
The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions.
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