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GATA3 Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IF - GATA3 Antibody (Center) AW5071-U100
    Fluorescent image of MCF-7 cells stained with GATA3 Antibody (Center)(Cat#AW5071). AW5071 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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  • WB - GATA3 Antibody (Center) AW5071-U100
    Western blot analysis of lysates from CEM, SH-SY5Y cell line (from left to right), using GATA3 Antibody (Center)(Cat. #AW5071). AW5071 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • FC - GATA3 Antibody (Center) AW5071-U100
    Flow cytometric analysis of MCF-7 cells using GATA3 Antibody (Center)(green, Cat#AW5071) compared to an isotype control of mouse IgG2b(blue). AW5071 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, WB
Primary Accession P23771
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=48 KDa
Isotype IgG2b,κ
Antigen Source HUMAN
Additional Information
Gene ID 2625
Other Names Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3
Dilution IF~~1:25
WB~~ 1:1000
FC~~1:25
Target/Specificity This GATA3 antibody is generated from a mouse immunized with a recombinant protein from human GATA3.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGATA3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GATA3
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.
Cellular Location Nucleus.
Tissue Location T-cells and endothelial cells.
Research Areas
Citations (0)
citation

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Background

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.

References

Joulin V.,et al.EMBO J. 10:1809-1816(1991).
Ho I.-C.,et al.EMBO J. 10:1187-1192(1991).
Ko L.J.,et al.Mol. Cell. Biol. 11:2778-2784(1991).
Marine J.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:7284-7288(1991).
Deloukas P.,et al.Nature 429:375-381(2004).

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$ 380.00
Cat# AW5071-U100
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