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HAND1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | O96004 |
| Other Accession | P57100, Q0VCE2, NP_004812.1, Q28555 |
| Reactivity | Human |
| Predicted | Bovine, Rabbit, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=24;Rat=24 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 9421 |
|---|---|
| Antigen Region | 76-105 aa |
| Other Names | HAND1; BHLHA27; EHAND; Heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1 |
| Dilution | WB~~1:1000 |
| Target/Specificity | This HAND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human HAND1. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HAND1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HAND1 |
|---|---|
| Synonyms | BHLHA27, EHAND |
| Function | Transcription factor that plays an essential role in both trophoblast giant cell differentiation and in cardiac morphogenesis (By similarity). Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity). Acts as a transcriptional repressor of SOX15 (By similarity). In the adult, could be required for ongoing expression of cardiac-specific genes (PubMed:9931445). |
| Cellular Location | Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity). |
| Tissue Location | Heart. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.
References
Reamon-Buettner, S.M., et al. Hum. Mol. Genet. 18(19):3567-3578(2009) Martinez Hoyos, J., et al. Oncogene 28(6):876-885(2009) Reamon-Buettner, S.M., et al. Hum. Mol. Genet. 17(10):1397-1405(2008) Morin, S., et al. J. Biol. Chem. 280(37):32272-32278(2005) Hill, A.A., et al. Mol. Cell. Biol. 24(22):9835-9847(2004)
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