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ACTA1/α-actin Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ACTA1/α-actin Antibody (C-term) AW5098-U100
    Western blot analysis of lysates from human normal uterus,human placenta,rat heart tissue lysate (from left to right), using ACTA1/α-actin (C-term)(Cat. #AW5098). AW5098 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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  • IHC - ACTA1/α-actin Antibody (C-term) AW5098-U100
    AW5098 staining ACTA1/alpha-actin in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P68133
Other Accession P60010, P68136, P68135, P68137, P68134, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P04751, P68035, P68033, P68032, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709, P48975
Reactivity Human, Rat
Predicted C.Elegans, Drosophila, Xenopus, Chicken, Bovine, Mouse, Rabbit, Zebrafish, Hamster, Horse, Monkey, Pig, Sheep, Yeast
Host Rabbit
Clonality Polyclonal
Calculated MW H=42;M=42;Rat=42 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 58
Antigen Region 189-217 aa
Other Names ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1
Dilution WB~~1:1000
IHC~~1:25
Target/Specificity This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTA1/α-actin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTA1
Synonyms ACTA
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)
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Background

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

References

Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) : Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010) Yu, G., et al. J Clin Neurosci 17(6):766-769(2010) Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010) : Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

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$ 370.00
Cat# AW5098-U100
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