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HADHA Antibody (C-term) [Knockout Validated]

Affinity Purified Rabbit Polyclonal Antibody (Pab) KO

     
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  • WB - HADHA Antibody (C-term) [Knockout Validated] AW5706-U100
    HADHA Antibody (C-term) (Cat.# AP6882b) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HADHA antibody detected the HADHA protein (arrow).
    detail
  • WB - HADHA Antibody (C-term) [Knockout Validated] AW5706-U100
    All lanes : Anti-HADHA Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - HADHA Antibody (C-term) [Knockout Validated] AW5706-U100
    A predominant 75 kDa band for the HEK293T wild type lysate was observed (3 ug/ml anti-HADHA) vs the predicted size of 83 kDa. The molecular weight discrepancy could be due to post-translationally modification of the target protein, a splice-variant form of the target protein, a partially degraded form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A less intense band at 65 kDa was also A weak band only in the 20ug lane was observed for the knock out lysate, suggesting incomplete knockout of the target gene.
    detail
  • IHC-P - HADHA Antibody (C-term) [Knockout Validated] AW5706-U100
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - HADHA Antibody (C-term) [Knockout Validated] AW5706-U100
    HADHA Antibody (C-term) (Cat. #AP6882b) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P
Primary Accession P40939
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 83000 Da
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 3030
Antigen Region 737-763 aa
Other Names Trifunctional enzyme subunit alpha, mitochondrial, 78 kDa gastrin-binding protein, TP-alpha, Long-chain enoyl-CoA hydratase, Long chain 3-hydroxyacyl-CoA dehydrogenase, HADHA, HADH
Dilution WB~~1:500-1:2000
IHC-P~~1:10~50
FC~~1:10~50
Target/Specificity This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-763 amino acids from the C-terminal region of human HADHA.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHADHA Antibody (C-term) [Knockout Validated] is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HADHA
Synonyms HADH
Function Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:8135828, PubMed:1550553, PubMed:29915090, PubMed:30850536). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed:29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed:30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity (PubMed:8135828, PubMed:29915090, PubMed:30850536). Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity (PubMed:23152787). It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP (PubMed:23152787). Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity (PubMed:23152787).
Cellular Location Mitochondrion. Mitochondrion inner membrane Note=Protein stability and association with mitochondrion inner membrane do not require HADHB.
Research Areas
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Background

HADHA is the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.

References

Sims,H.F., et.al., Proc. Natl. Acad. Sci. U.S.A. 92 (3), 841-845 (1995)

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$ 370.00
Cat# AW5706-U100
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