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DANRE actba Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DANRE actba Antibody (Center) Azb10021c
    DANRE actba Antibody (Center) (Cat. #Azb10021c) western blot analysis in zebra fish muscle tissue lysates (35ug/lane).This demonstrates the DANRE actba antibody detected the DANRE actba protein (arrow).
    detail
  • WB - DANRE actba Antibody (Center) Azb10021c
    All lanes : Anti-DANRE actba Antibody (Center) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7ZVI7
Other Accession P60010, P68136, P68135, P68137, P68134, P68133, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P04751, P68035, P68033, P68032, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709
Reactivity Zebrafish
Predicted C.Elegans, Drosophila, Xenopus, Chicken, Bovine, Human, Mouse, Rabbit, Rat, Hamster, Horse, Monkey, Pig, Sheep, Yeast
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41767 Da
Antigen Region 188-215 aa
Additional Information
Gene ID 57934
Other Names Actin, cytoplasmic 1, Beta-actin-1, Actin, cytoplasmic 1, N-terminally processed, actba, bact, bactin1, bactzf
Target/Specificity This DANRE actba antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of DANRE actba.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE actba Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name actba
Synonyms bact, bactin1, bactzf
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Cellular Location Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P60710}. Nucleus {ECO:0000250|UniProtKB:O93400}
Tissue Location Skeletal muscle, heart, gill, digestive tissue and brain (PubMed:9987040). Widespread expression throughout the brain, with highest levels in regions where neuronal proliferation is greatest (PubMed:9987040).
Research Areas
Citations (0)
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Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

References

Barrallo A., et al. Eur. J. Neurosci. 11:369-372(1999).

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$ 365.00
$ 140.00
Cat# Azb10021c
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