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Zebrafish tcf21 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q32PV5 |
| Reactivity | Zebrafish |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 19346 Da |
| Antigen Region | 5-35 aa |
| Gene ID | 558148 |
|---|---|
| Other Names | Transcription factor 21 {ECO:0000312|EMBL:AAI079691}, TCF-21, Capsulin {ECO:0000312|EMBL:ABU954061}, Epicardin, MyoRa2, TCF21 |
| Target/Specificity | This Zebrafish tcf21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of zebrafish tcf21. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Zebrafish tcf21 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TCF21 |
|---|---|
| Function | Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:O43680, ECO:0000255|PROSITE-ProRule:PRU00981} |
| Tissue Location | Expressed in the cranial paraxial mesoderm from 20 hpf and subsequently becomes restricted to the pharyngeal mesoderm that will form the muscle. Expression in the proepicardial organ is first seen at 40hpf in a cluster of cells between the myocardium and yolk Also expressed in the developing arches. Expression begins to surround the heart by day 3 of development, and by 96 hpf, expression is restricted to the outer epicardial layer surrounding the myocardium |
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Provided below are standard protocols that you may find useful for product applications.
Background
Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis (By similarity).
References
Wang Y., et al. J. Mol. Evol. 68:629-640(2009).
Chang M.-Y., et al. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
Serluca F.C., et al. Dev. Biol. 315:18-27(2008).
Knight R.D., et al. J. Exp. Zool. B Mol. Dev. Evol. 310:355-369(2008).
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