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DANRE tcf21 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DANRE tcf21 Antibody (N-term) Azb10030a
    DANRE tcf21 Antibody (N-term) (Cat. #Azb10030a) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the DANRE tcf21 antibody detected the DANRE tcf21 protein (arrow).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q32PV5
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 5-35 aa
Additional Information
Other Names Transcription factor 21 {ECO:0000312|EMBL:AAI079691}, TCF-21, Capsulin {ECO:0000312|EMBL:ABU954061}, Epicardin, MyoRa2, TCF21
Target/Specificity This DANRE tcf21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-35 amino acids from the N-terminal region of DANRE tcf21.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE tcf21 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCF21
Function Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis.
Cellular Location Nucleus {ECO:0000250|UniProtKB:O43680, ECO:0000255|PROSITE-ProRule:PRU00981}
Tissue Location Expressed in the cranial paraxial mesoderm from 20 hpf and subsequently becomes restricted to the pharyngeal mesoderm that will form the muscle. Expression in the proepicardial organ is first seen at 40hpf in a cluster of cells between the myocardium and yolk Also expressed in the developing arches. Expression begins to surround the heart by day 3 of development, and by 96 hpf, expression is restricted to the outer epicardial layer surrounding the myocardium EMBL; EF632300; ABU95406.1; -; mRNA EMBL; BC107968; AAI07969.1; -; mRNA RefSeq; NP_001032770.1; NM_001037681.1 SMR; Q32PV5; - STRING; 7955.ENSDARP00000053538; - PaxDb; Q32PV5; - GeneID; 558148; - KEGG; dre:558148; - CTD; 6943; - ZFIN; ZDB-GENE-051113-88; tcf21 eggNOG; KOG4029; Eukaryota InParanoid; Q32PV5; - OrthoDB; 1374128at2759; - PhylomeDB; Q32PV5; - PRO; PR:Q32PV5; - Proteomes; UP000000437; Unplaced GO; GO:0005634; C:nucleus; IDA:UniProtKB GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISS:UniProtKB GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISS:UniProtKB GO; GO:0070888; F:E-box binding; ISS:UniProtKB GO; GO:0046983; F:protein dimerization activity; ISS:UniProtKB GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central GO; GO:0060840; P:artery development; IGI:ZFIN GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; ISS:UniProtKB GO; GO:0014707; P:branchiomeric skeletal muscle development; IMP:ZFIN GO; GO:0060435; P:bronchiole development; ISS:UniProtKB GO; GO:0060539; P:diaphragm development; ISS:UniProtKB GO; GO:0048557; P:embryonic digestive tract morphogenesis; ISS:UniProtKB GO; GO:0030855; P:epithelial cell differentiation; ISS:UniProtKB GO; GO:0048732; P:gland development; ISS:UniProtKB GO; GO:0032835; P:glomerulus development; ISS:UniProtKB GO; GO:0001822; P:kidney development; ISS:UniProtKB GO; GO:0048286; P:lung alveolus development; ISS:UniProtKB GO; GO:0060425; P:lung morphogenesis; ISS:UniProtKB GO; GO:0060426; P:lung vasculature development; ISS:UniProtKB GO; GO:0072277; P:metanephric glomerular capillary formation; ISS:UniProtKB GO; GO:0072162; P:metanephric mesenchymal cell differentiation; ISS:UniProtKB GO; GO:0001763; P:morphogenesis of a branching structure; ISS:UniProtKB GO; GO:0060766; P:negative regulation of androgen receptor signaling pathway; ISS:UniProtKB GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central GO; GO:0048608; P:reproductive structure development; ISS:UniProtKB GO; GO:0060541; P:respiratory system development; ISS:UniProtKB GO; GO:0060021; P:roof of mouth development; ISS:UniProtKB GO; GO:0060008; P:Sertoli cell differentiation; ISS:UniProtKB GO; GO:0007530; P:sex determination; ISS:UniProtKB GO; GO:0007519; P:skeletal muscle tissue development; IMP:ZFIN GO; GO:0048536; P:spleen development; ISS:UniProtKB GO; GO:0001657; P:ureteric bud development; ISS:UniProtKB GO; GO:0001944; P:vasculature development; ISS:UniProtKB CDD; cd00083; HLH; 1 Gene3D; 4.10.280.10; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf Pfam; PF00010; HLH; 1 SMART; SM00353; HLH; 1 SUPFAM; SSF47459; SSF47459; 1 PROSITE; PS50888; BHLH; 1 2: Evidence at transcript level; Differentiation; DNA-binding; Nucleus; Reference proteome; Transcription; Transcription regulation CHAIN 1..176 /note="Transcription factor 21" /id="PRO_0000386430" DOMAIN 76..128 /note="bHLH" /evidence="ECO:0000255|PROSITE-ProRule:PRU00981" SEQUENCE 176 AA; 19346 MW; AD05C77D86290C55 CRC64; MSTGSISDVD EFHESELLDG LPKFGSGKDP GTSNESTEDS SNCEGASVSE CTGKRRKSAN MRRSAPNGVA QEGKQVQRNA ANARERARMR VLSKAFSRLK TTLPWVPPDT KLSKLDTLRL ASSYIAHLRQ ILANDKYENG YIHPVNLTWP FMVAGKPENE LKEMLNSTRL CGTTAS
Research Areas
Citations (0)

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Background

Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis (By similarity).

References

Wang Y., et al. J. Mol. Evol. 68:629-640(2009).
Chang M.-Y., et al. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
Serluca F.C., et al. Dev. Biol. 315:18-27(2008).
Knight R.D., et al. J. Exp. Zool. B Mol. Dev. Evol. 310:355-369(2008).

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Cat# Azb10030a
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