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SPOP Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q6ZWS8 |
| Other Accession | Q7T330 |
| Reactivity | Mouse, Zebrafish |
| Predicted | Human, Bovine, Drosophila, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone/Animal Names | RB49885 |
| Calculated MW | 42132 Da |
| Gene ID | 20747 |
|---|---|
| Other Names | Speckle-type POZ protein, HIB homolog 1, SPOP1, spop |
| Target/Specificity | This SPOP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 74-107 amino acids from the N-terminal region of mouse SPOP. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SPOP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Spop {ECO:0000312|EMBL:AAT08952.1} |
|---|---|
| Synonyms | Pcif1 |
| Function | Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL (By similarity). In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. Involved in the regulation of bromodomain and extra-terminal motif (BET) proteins BRD2, BRD3, BRD4 stability (By similarity). Plays an essential role for proper translation, but not for their degradation, of critical DNA replication licensing factors CDT1 and CDC6, thereby participating in DNA synthesis and cell proliferation. Regulates interferon regulatory factor 1/IRF1 proteasomal turnover by targeting S/T-rich degrons in IRF1 (By similarity). Involved in ubiquitination of BRDT and promotes its degradation, thereby regulates histone removal in early condensing spermatids prior to histone-to-protamine exchange (PubMed:32726616). |
| Cellular Location | Nucleus. Nucleus speckle |
| Tissue Location | Widely expressed, mainly in pancreas and in particular in adult pancreatic insulin-producing beta cells and in a subset of exocrine acinar and duct cells |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation (By similarity).
References
Zhang Q.,et al.Dev. Cell 10:719-729(2006).
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$ 385.00
$ 150.00
Cat# Azb18701a
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