SPN/CD43 Antibody (Center) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P16150 |
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Clone Names | 101229058 |
Gene ID | 6693 |
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Other Names | Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43, SPN, CD43 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP14085c was selected from the Center region of SPN/CD43. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | SPN |
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Synonyms | CD43 |
Function | Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E- selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity). |
Cellular Location | Membrane; Single-pass type I membrane protein. Cell projection, microvillus {ECO:0000250|UniProtKB:P13838}. Cell projection, uropodium {ECO:0000250|UniProtKB:P15702}. Note=Localizes to the uropodium and microvilli via its interaction with ERM proteins (EZR, RDX and MSN) {ECO:0000250|UniProtKB:P13838, ECO:0000250|UniProtKB:P15702} |
Tissue Location | Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas |
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Provided below are standard protocols that you may find useful for product applications.
Background
Sialophorin (leukosialin) is a major sialoglycoprotein onthe surface of human T lymphocytes, monocytes, granulocytes, andsome B lymphocytes, which appears to be important for immunefunction and may be part of a physiologic ligand-receptor complexinvolved in T-cell activation.
References
Urano-Tashiro, Y., et al. Infect. Immun. 76(10):4686-4691(2008)Mambole, A., et al. J. Biol. Chem. 283(35):23627-23635(2008)Seethala, R.R., et al. Appl. Immunohistochem. Mol. Morphol. 16(2):165-172(2008)Khunkaewla, P., et al. Mol. Immunol. 45(6):1703-1711(2008)Rawal, A., et al. Arch. Pathol. Lab. Med. 131(11):1673-1678(2007)
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