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PHF22 Antibody (N-term T88) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q96CB8 |
|---|---|
| Clone Names | 5082552 |
| Gene ID | 57117 |
|---|---|
| Other Names | Integrator complex subunit 12, Int12, PHD finger protein 22, INTS12, PHF22 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP1912a was selected from the N-term region of human PHF22. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | INTS12 {ECO:0000303|PubMed:38570683, ECO:0000312|HGNC:HGNC:25067} |
|---|---|
| Function | Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:38570683). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C- terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed:38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed:16239144). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed:23904267). |
| Cellular Location | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
PHF22 is a component of the integrator complex stably associated with RNA polymerase II. The integrator complex mediates RNA polymerase-II-dependent transcription. This protein is recruited to the U1 and U2 snRNA genes and mediates the snRNA's 3' end processing.
References
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
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