|Other Names||Histone-lysine N-methyltransferase mes-4, Maternal-effect sterile protein 4, mes-4|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1965b was selected from the C-term region of human Mes-4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Histone methyltransferase. Dimethylates 'Lys-36' of histone H3, a specific tag for epigenetic transcriptional activation. Plays a central role in early development and is responsible for all H3 'Lys- 36' dimethylation until about the 40-cell stage. Indirectly involved in the global inactivation of the X chromosomes in germline cells, possibly by excluding the mes-2-mes-3-mes-6 repressive Polycomb complex from the autosomes (PubMed:12077420, PubMed:16968818). Not related to transcription elongation (PubMed:12077420, PubMed:16968818). Required for small-RNA-induced H3K27 trimethylation (PubMed:26365259). May suppress sensitivity to RNAi (PubMed:16507136). May regulate the expression of genes required for vulval development (PubMed:16507136, PubMed:16710447).|
|Cellular Location||Nucleus. Chromosome. Note=Specifically associated with the autosomes and with the distal tip of chromosome X. Colocalizes with methylated 'Lys-4' of histone H3|
|Tissue Location||In adults, it is predominantly expressed in the germline, and weakly expressed in intestinal cells|
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Provided below are standard protocols that you may find useful for product applications.
Mes-4 is a histone methyltransferase. This protein dimethylates 'Lys-36' of histone H3, a specific tag for epigenetic transcriptional activation. Mes-4 plays a central role in early development and is responsible for all H3 'Lys-36' dimethylation until about the 40-cell stage. It appears to be indirectly involved in the global inactivation of the X chromosomes in germ line cells, possibly by excluding the mes-2-mes-3-mes-6 repressive Polycomb complex from the autosomes.
Bender L.B., et al. Development. 2006. 133:3907-3917.Fong Y, et al. Science. 2002. 296(5576):2235-8.Korf I, et al. Development. 1998. 125(13):2469-78. Kelly WG, et al. Development. 1998. 125(13):2451-6.Garvin C, et al. Genetics. 1998. 148(1):167-85.
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