|Other Names||Uncharacterized protein F54F22, isoform a, F54F22/F54F23/F54F24|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1972a was selected from the N-term region of human Cezf. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Recruits the histone methyltransferase dot-1.1 to chromatin to methylate 'Lys-79' of histone H3 and activate transcription (PubMed:23806335). Recognizes and binds histone H3 methylated at 'Lys- 4' (H3K4me) at the promoters of target genes (PubMed:23263989). During stress, the zfp-1-dot-1.1 complex also plays a role in the deubiquitination of histone H2B sites, which negatively modulates the RNA polymerase II-induced transcription of highly expressed genes (PubMed:23806335). In response to stress, binds to the pdk-1 promoter to negatively regulate pdk-1 expression, which negatively modulates daf-16/FOXO-mediated gene expression (PubMed:21980302). Thus, most likely via this mechanism, in response to stress, it confers a protective role against neuronal necrosis (PubMed:25422944). Plays a role in Insulin/IGF-1-like signaling (IIS)- and diet restriction- mediated lifespan extension by controlling daf-16/FOXO and pha-4/FOXA recruitment to target promoters (PubMed:27039057). May negatively regulate the expression of genes required for vulval development (PubMed:16507136, PubMed:16710447). May play a role in axon guidance in D-type motor neurons (PubMed:24066155). May suppress sensitivity to RNAi (PubMed:16507136).|
|Cellular Location||Nucleus. Chromosome Note=Localizes to all six condensed chromosomes (PubMed:23263989). zfp- 1 and dot-1.1 colocalize to promoters of highly expressed genes (PubMed:23806335).|
|Tissue Location||Isoform a: Expressed at high levels in maturing oocytes, but at low levels in the rest of the germ line (at protein level) (PubMed:23263989). Isoform a: Not expressed in the pharynx, germ line and tail (PubMed:27039057). Isoform c: Not expressed in the germ line (at protein level) (PubMed:23263989). Isoform c: Uniformly expressed (PubMed:27039057).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
CEZF bears substantial homology ot the zinc finger and leucine zipper motifs of the human gene products AF10, MLLT6, and BR140. The functional domain of the zing finger motif, which is structurally different from previously described zinc finger motifs, is potentially disrupted in leukemia-associated chromosomal translocations. This zinc finger motif present in CEZF, called the leukemia-associatged protein (LAP) finger, is an important DNA-binding domain in this group of regulatory proteins, and may be involved in leukemogenesis.
Saha, V, et al. PNAS USA. 1995. 92(21): 9737锟?741.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.