|Other Names||Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2113b was selected from the C-term region of human UBE1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).|
|Cellular Location||Cytoplasm. Mitochondrion. Nucleus [Isoform 2]: Cytoplasm|
|Tissue Location||Detected in erythrocytes (at protein level). Ubiquitous.|
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Provided below are standard protocols that you may find useful for product applications.
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.
Ayusawa, D., et al., Cell Struct. Funct. 17(2):113-122 (1992).Handley, P.M., et al., Proc. Natl. Acad. Sci. U.S.A. 88(1):258-262 (1991).Kudo, M., et al., Exp. Cell Res. 192(1):110-117 (1991).Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53(1):20-22 (1990).Zacksenhaus, E., et al., EMBO J. 9(9):2923-2929 (1990).
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