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GGA3 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
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- BACKGROUND
| Primary Accession | Q9NZ52 |
|---|---|
| Other Accession | GGA3_HUMAN |
| Clone Names | 3103102 |
| Gene ID | 23163 |
|---|---|
| Other Names | ADP-ribosylation factor-binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, GGA3, KIAA0154 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2318b was selected from the C-term region of human GGA3 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | GGA3 {ECO:0000312|MIM:606006} |
|---|---|
| Synonyms | KIAA0154 |
| Function | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed:11301005). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed:26811329). nvolved in BACE1 transport and sorting as well as regulation of BACE1 protein levels (PubMed:15615712, PubMed:17553422, PubMed:20484053). Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation (PubMed:15615712). Modulates BACE1 protein levels independently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination (PubMed:20484053). Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane (By similarity). |
| Cellular Location | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane {ECO:0000250|UniProtKB:A0A0G2JV04}; Peripheral membrane protein |
| Tissue Location | Ubiquitously expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
GGA3 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.
References
Wakasugi, M., et al., Biochem. Biophys. Res. Commun. 306(3):687-692 (2003).Misra, S., et al., Nature 415(6874):933-937 (2002).Puertollano, R., et al., Science 292(5522):1712-1716 (2001).Takatsu, H., et al., Biochem. Biophys. Res. Commun. 271(3):719-725 (2000).Dell'Angelica, E.C., et al., J. Cell Biol. 149(1):81-94 (2000).
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