CDT1 Antibody (Center) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9H211 |
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Clone Names | 80317143 |
Gene ID | 81620 |
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Other Names | DNA replication factor Cdt1, Double parked homolog, DUP, CDT1 {ECO:0000312|EMBL:AAG451811} |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2952c was selected from the Center region of human CDT1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | CDT1 {ECO:0000312|EMBL:AAG45181.1} |
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Function | Required for both DNA replication and mitosis (PubMed:11125146, PubMed:22581055, PubMed:21856198, PubMed:14993212, PubMed:26842564). DNA replication licensing factor, required for pre- replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed:14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity). |
Cellular Location | Nucleus. Chromosome, centromere, kinetochore. Note=Transiently localizes to kinetochores during prometaphase and metaphase |
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Provided below are standard protocols that you may find useful for product applications.
Background
CDT1 Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It Binds DNA in a sequence-, strand-, and conformation-independent manner.
References
Whittaker,A.J., et.al., Genes Dev. 14 (14), 1765-1776 (2000)Nishitani,H., et.al., Nature 404 (6778), 625-628 (2000)Herbig,U., et.al., Mol. Biol. Cell 10 (8), 2631-2645 (1999)
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