Phospho-BRAF(S601) Antibody Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P15056 |
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Clone Names | 110020 |
Gene ID | 673 |
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Other Names | Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP3373a was selected from the region of human Phospho-BRAF-S602. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | BRAF (HGNC:1097) |
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Synonyms | BRAF1, RAFB1 |
Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179). |
Cellular Location | Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. |
Tissue Location | Brain and testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.
References
Loewe, R., et al., J. Invest. Dermatol. 123(4):733-736 (2004).Yamaguchi, T., et al., J. Biol. Chem. 279(39):40419-40430 (2004).Frattini, M., et al., Oncogene 23(44):7436-7440 (2004).Tsavachidou, D., et al., Cancer Res. 64(16):5556-5559 (2004).Gear, H., et al., Invest. Ophthalmol. Vis. Sci. 45(8):2484-2488 (2004).
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