AF4 (MLLT2) Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P51825 |
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Clone Names | 4091609 |
Gene ID | 4299 |
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Other Names | AF4/FMR2 family member 1, ALL1-fused gene from chromosome 4 protein, Protein AF-4, Protein FEL, Proto-oncogene AF4, AFF1, AF4, FEL, MLLT2, PBM1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6189b was selected from the N-term region of human MLLT2 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | AFF1 |
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Synonyms | AF4, FEL, MLLT2, PBM1 |
Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
MLLT2 is involved in acute leukemias through a chromosomal translocation t(4;11)(q21;q23) that involves mllt2 and mll/hrx. AF-4 (MLLT2), AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains. AF4 is a serine- and proline-rich putative transcription factor with a glutamine-rich carboxyl terminus. The composition of the complete MLL-AF4 fusion product argues that it may act through either a gain-of-function or a dominant negative mechanism in leukemogenesis.
References
Bursen, A., et al., Oncogene 23(37):6237-6249 (2004).Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).Caslini, C., et al., Leukemia 18(6):1064-1071 (2004).Bertrand, F.E., et al., Leukemia 17(12):2454-2459 (2003).Reichel, M., et al., Leukemia 15(2):286-288 (2001).
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